The show - based on the novels of Sophie Hannah - followed cops DS Charlie Zailer (Olivia Williams) and DC Simon Waterhouse (Darren Boyd).
Boyd broke the news of the show's axe on Twitter, writing: "Those of you who enjoyed Case Sensitive, I'm sorry to report that ITV have decided not to make any more. Shame, but them's the breaks."
The actor later tweeted at author Hannah: "A real shame we won't get to explore Charlie & Simon anymore. Was a pleasure and a joy working with you."
The first series of Case Sensitive attracted an average of 5.64m viewers in 2011, while the two-part second run averaged 4.35m.
Last month, Boyd told that there was "huge scope" for future series of Case Sensitive.
"We've got cases and storylines coming out of our ears, thanks to Sophie, we've got a wealth of material there," he said.
"If things go well and people like [series two] as much as the first one - and I see no reason why they shouldn't - we could be doing this for a while and I would be very happy if we did that."
